PrimePCR™ Probe Assay: LPAR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: LPAR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LPAR1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026307
Assay Design:   Intron-spanning
Chromosome Location:   9:113637919-113703787question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000374431 ENST00000374430 ENST00000358883 ENST00000538760 ENST00000541779 ENST00000449490

Gene Information

The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling EDG receptors mediate diverse biologic functions including proliferation platelet aggregation smooth muscle contraction inhibition of neuroblastoma cell differentiation chemotaxis and tumor cell invasion. Two transcript variants encoding the same protein have been identified for this gene [provided by RefSeq Jul 2008]

Gene Symbol:   LPAR1
Gene Name:   lysophosphatidic acid receptor 1
Aliases:   EDG2, GPR26, Gpcr26, LPA1, Mrec1.3, VZG1, edg-2, rec.1.3, vzg-1
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000198121
Entrez:   1902
Chromosome Mapping:   9q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.850000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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