PrimePCR™ Probe Assay: UBA5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026302
Assay Design:   Intron-spanning
Chromosome Location:   3:132387706-132389113question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000356232 ENST00000493720 ENST00000473651 ENST00000494238 ENST00000464068 ENST00000489361 ENST00000264991

Gene Information

This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1 a ubiquitin-like post-translational modifier protein via the formation of a high-energy thioester bond. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A pseudogene located on chromosome 1 has also been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   UBA5
Gene Name:   ubiquitin-like modifier activating enzyme 5
Aliases:   FLJ17281, FLJ23251, FLJ23251UBA5, THIFP1, UBE1DC1
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000081307
Entrez:   79876
UniGene:   Hs.170737
Chromosome Mapping:   3q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 34.320000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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