PrimePCR™ Probe Assay: GPBP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026216
Assay Design:   Intron-spanning
Chromosome Location:   5:56557058-56558548question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000424459 ENST00000514387 ENST00000506184 ENST00000511209 ENST00000264779 ENST00000454432 ENST00000538707

Gene Information

This gene was originally isolated by subtractive hybridization of cDNAs expressed in atherosclerotic plaques with a thrombus and was found to be expressed only in vascular smooth muscle cells. However a shorter splice variant was found to be more ubiquitously expressed. This protein is suggested to play a role in the development of atherosclerosis. Studies in mice suggest that it may also function as a GC-rich promoter-specific trans-activating transcription factor. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Feb 2011]

Gene Symbol:   GPBP1
Gene Name:   GC-rich promoter binding protein 1
Aliases:   DKFZp761C169, GPBP, MGC126339, SSH6, VASCULIN
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000062194
Entrez:   65056
UniGene:   Hs.444279
Chromosome Mapping:   5q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.630000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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