PrimePCR™ Probe Assay: FNDC3A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026173
Assay Design:   Intron-spanning
Chromosome Location:   13:49772635-49775981question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000492622 ENST00000337156 ENST00000398316 ENST00000541916

Gene Information

Description Not Available

Gene Symbol:   FNDC3A
Gene Name:   fibronectin type III domain containing 3A
Aliases:   FLJ31509, FNDC3, HUGO, KIAA0970, bA203I16.1, bA203I16.5
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000102531
Entrez:   22862
UniGene:   Hs.508010
Chromosome Mapping:   13q14.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.260000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download