PrimePCR™ Probe Assay: SMAD9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026117
Assay Design:   Intron-spanning
Chromosome Location:   13:37427750-37439765question
Amplicon Length:   125
Splice Variants Targeted:   ENST00000399275 ENST00000350148 ENST00000379826

Gene Information

The protein encoded by this gene is a member of the SMAD family which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Jan 2010]

Gene Symbol:   SMAD9
Gene Name:   SMAD family member 9
Aliases:   MADH6, MADH9, SMAD8, SMAD8A, SMAD8B
RefSeq:   NC_000013.10 NG_016963.1 NT_024524.14
Ensembl:   ENSG00000120693
Entrez:   4093
UniGene:   Hs.123119
Chromosome Mapping:   13q12-q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.700000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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