PrimePCR™ Probe Assay: MORC3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026025
Assay Design:   Intron-spanning
Chromosome Location:   21:37744760-37747506question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000400485

Gene Information

This gene encodes a protein that localizes to the nuclear matrix. The protein also has RNA binding activity and has a predicted coiled-coil domain. [provided by RefSeq Jul 2008]

Gene Symbol:   MORC3
Gene Name:   MORC family CW-type zinc finger 3
Aliases:   NXP2, ZCW5, ZCWCC3
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000159256
Entrez:   23515
UniGene:   Hs.421150
Chromosome Mapping:   21q22.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.450000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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