PrimePCR™ Probe Assay: SMARCC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0025971
Assay Design:   Intron-spanning
Chromosome Location:   3:47651779-47663818question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000254480

Gene Information

The protein encoded by this gene is a member of the SWI/SNF family of proteins whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. [provided by RefSeq Jul 2008]

Gene Symbol:   SMARCC1
Gene Name:   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
Aliases:   BAF155, CRACC1, Rsc8, SRG3, SWI3
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000173473
Entrez:   6599
UniGene:   Hs.476179
Chromosome Mapping:   3p21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.440000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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