This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0041865
PrimePCR™ PreAmp for SYBR® Green Assay: TBC1D3C, Human
PrimePCR™ Template for SYBR® Green Assay: TBC1D3C, Human
This gene represents one of at least 8 paralogs of TBC1D3 gene found on chromosome 17. These paralogs appear to be differentially expressed in human tissues. The proteins encoded by this gene family contain a TBC (Tre-2 Bub2p and Cdc16p) domain which is found in proteins involved in RAB GTPase signaling and vesicle trafficking. [provided by RefSeq May 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.