PrimePCR™ SYBR® Green Assay: ZNF880, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041537

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038815
Assay Design:   Intron-spanning
Chromosome Location:   19:52873204-52877598question
Amplicon Length:   171
Splice Variants Targeted:   ENST00000595962 ENST00000598016 ENST00000424032 ENST00000600321 ENST00000344085 ENST00000597976 ENST00000422689 ENST00000423902 ENST00000251287 ENST00000405415 ENST00000404406 ENST00000337579 ENST00000441023 ENST00000539903 ENST00000403107 ENST00000404077 ENST00000297186 ENST00000433859 ENST00000359718

Gene Information

Description Not Available

Gene Symbol:   ZNF880
Gene Name:   zinc finger protein 880
Aliases:   Not Available
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000221923
Entrez:   400713
UniGene:   Hs.502314
Chromosome Mapping:   19q13.41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.020000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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