PrimePCR™ SYBR® Green Assay: FAM22G, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041501

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038779
Assay Design:   Intron-spanning
Chromosome Location:   9:99697677-99698768question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000451286 ENST00000381689 ENST00000381707 ENST00000412718 ENST00000381697 ENST00000372321 ENST00000342531 ENST00000448135 ENST00000429828 ENST00000602967 ENST00000429984 ENST00000354649 ENST00000372322 ENST00000253262 ENST00000335456 ENST00000341207

Gene Information

Description Not Available

Gene Symbol:   FAM22G
Gene Name:   family with sequence similarity 22, member G
Aliases:   Not Available
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000188152
Entrez:   441457
Chromosome Mapping:   9q22.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.920000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download