PrimePCR™ SYBR® Green Assay: DHRS4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041443

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DHRS4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DHRS4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038721
Assay Design:   Intron-spanning
Chromosome Location:   14:24423072-24424327question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000322475 ENST00000313250 ENST00000558263 ENST00000543741 ENST00000397075 ENST00000397074 ENST00000559632 ENST00000558581 ENST00000421831 ENST00000397073 ENST00000308178 ENST00000382761 ENST00000397071 ENST00000559411 ENST00000335125 ENST00000558753 ENST00000537912 ENST00000545240 ENST00000382755 ENST00000568908 ENST00000568909 ENST00000567835 ENST00000562631 ENST00000388813 ENST00000562558 ENST00000540164 ENST00000388812 ENST00000538815 ENST00000456916 ENST00000379694 ENST00000379696 ENST

Gene Information

Description Not Available

Gene Symbol:   DHRS4
Gene Name:   dehydrogenase/reductase (SDR family) member 4
Aliases:   CR, FLJ11008, NRDR, PHCR, PSCD, SCAD-SRL, SDR-SRL, SDR25C1, SDR25C2
RefSeq:   NC_000014.8 NG_023545.1 NT_026437.12
Ensembl:   ENSG00000157326
Entrez:   10901
Chromosome Mapping:   14q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.970000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download