PrimePCR™ SYBR® Green Assay: DEF8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041426

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DEF8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DEF8, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038704
Assay Design:   Intron-spanning
Chromosome Location:   16:90020752-90021668question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000563594 ENST00000567999 ENST00000566079 ENST00000566820 ENST00000562578 ENST00000561741 ENST00000268676 ENST00000569453 ENST00000567884 ENST00000569061 ENST00000418391 ENST00000561959 ENST00000570182 ENST00000563795 ENST00000564836 ENST00000260795 ENST00000352904 ENST00000340107 ENST00000440486 ENST00000412135 ENST00000283426 ENST00000502646 ENST00000398181

Gene Information

Description Not Available

Gene Symbol:   DEF8
Gene Name:   differentially expressed in FDCP 8 homolog (mouse)
Aliases:   FLJ20186, MGC104349
RefSeq:   NC_000016.9 NT_010542.15
Ensembl:   ENSG00000140995
Entrez:   54849
Chromosome Mapping:   16q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.600000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download