PrimePCR™ SYBR® Green Assay: PIN4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041383

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PIN4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PIN4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038661
Assay Design:   Intron-spanning
Chromosome Location:   X:71406311-71416734question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000218432 ENST00000373669 ENST00000496835 ENST00000446576 ENST00000423432 ENST00000336092

Gene Information

This gene encodes a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. The encoded protein catalyzes the isomerization of peptidylprolyl bonds and may play a role in the cell cycle chromatin remodeling and/or ribosome biogenesis. The encoded protein may play an additional role in the mitochondria. [provided by RefSeq Dec 2009]

Gene Symbol:   PIN4
Gene Name:   protein (peptidylprolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin)
Aliases:   EPVH, MGC138486, PAR14, PAR17
RefSeq:   NC_000023.10 NT_011669.17 NG_016328.1
Ensembl:   ENSG00000102309
Entrez:   5303
Chromosome Mapping:   Xq13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.760000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download