PrimePCR™ SYBR® Green Assay: ZNF730, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040954

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF730, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF730, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038232
Assay Design:   Intron-spanning
Chromosome Location:   19:23316894-23318834question
Amplicon Length:   170
Splice Variants Targeted:   ENST00000324198 ENST00000282869 ENST00000328747 ENST00000591366 ENST00000592160 ENST00000343769 ENST00000427401 ENST00000596797 ENST00000594363 ENST00000594947 ENST00000600162 ENST00000601693 ENST00000357491 ENST00000357774 ENST00000593657 ENST00000601553 ENST00000456783 ENST00000596209 ENST00000397104 ENST00000593635 ENST00000597761 ENST00000571873 ENST00000596562 ENST00000570694

Gene Information

Description Not Available

Gene Symbol:   ZNF730
Gene Name:   zinc finger protein 730
Aliases:   Not Available
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000183850
Entrez:   100129543
Chromosome Mapping:   19p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.730000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download