PrimePCR™ SYBR® Green Assay: GTF2IRD2B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040904

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GTF2IRD2B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GTF2IRD2B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038182
Assay Design:   Intron-spanning
Chromosome Location:   7:74554805-74558323question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000324896 ENST00000353920 ENST00000346152 ENST00000416070 ENST00000405086 ENST00000361071 ENST00000453619 ENST00000356115 ENST00000312575 ENST00000394939 ENST00000430511 ENST00000573035

Gene Information

This gene encodes a glycosylated phosphoprotein with a leucine zipper motif two helix-loop-helix motifs (I repeats) that are similar to domains found in the TFII-I family of transcription factors one CHARLIE8 transposable element-like sequence and a BED zinc finger. This gene lies within a region that is deleted in Williams-Beuren syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however not all variants have been fully characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   GTF2IRD2B
Gene Name:   GTF2I repeat domain containing 2B
Aliases:   Not Available
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000174428
Entrez:   389524
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.630000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download