This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0040904
PrimePCR™ PreAmp for SYBR® Green Assay: GTF2IRD2B, Human
PrimePCR™ Template for SYBR® Green Assay: GTF2IRD2B, Human
This gene encodes a glycosylated phosphoprotein with a leucine zipper motif two helix-loop-helix motifs (I repeats) that are similar to domains found in the TFII-I family of transcription factors one CHARLIE8 transposable element-like sequence and a BED zinc finger. This gene lies within a region that is deleted in Williams-Beuren syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however not all variants have been fully characterized. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.