PrimePCR™ SYBR® Green Assay: PHB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040856

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PHB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PHB, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038133
Assay Design:   Intron-spanning
Chromosome Location:   17:47489126-47492204question
Amplicon Length:   168
Splice Variants Targeted:   ENST00000355202 ENST00000395835 ENST00000300408 ENST00000419140 ENST00000504124 ENST00000512041 ENST00000446735 ENST00000434917 ENST00000511832 ENST00000274054

Gene Information

Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq Jul 2008]

Gene Symbol:   PHB
Gene Name:   prohibitin
Aliases:   PHB1
RefSeq:   NC_000017.10 NG_023046.1 NT_010783.15
Ensembl:   ENSG00000167085
Entrez:   5245
Chromosome Mapping:   17q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996100
y-intercept 36.850000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download