PrimePCR™ SYBR® Green Assay: SSX3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040849

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SSX3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SSX3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038126
Assay Design:   Intron-spanning
Chromosome Location:   X:48209444-48211563question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000381337 ENST00000431224 ENST00000429387 ENST00000381621 ENST00000381623 ENST00000298181 ENST00000336777 ENST00000337502 ENST00000375515 ENST00000276049 ENST00000328439 ENST00000536311 ENST00000037502 ENST00000412716 ENST00000549555 ENST00000425012 ENST00000551313 ENST00000376932 ENST00000319275 ENST00000376923 ENST00000311798 ENST00000403001 ENST00000347757 ENST00000376919 ENST00000376909 ENST00000407081 ENST00000298396 ENST00000376895 ENST00000376893 ENST00000376886 ENST00000376884 ENST

Gene Information

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1 SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This gene appears not to be involved in this type of chromosome translocation. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   SSX3
Gene Name:   synovial sarcoma, X breakpoint 3
Aliases:   CT5.3, MGC119054, MGC14495
RefSeq:   NC_000023.10 NG_012561.1 NT_079573.4
Ensembl:   ENSG00000165584
Entrez:   10214
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.540000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download