PrimePCR™ SYBR® Green Assay: METTL2B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040846

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038123
Assay Design:   Intron-spanning
Chromosome Location:   7:128119541-128128197question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000311506 ENST00000462662 ENST00000262432 ENST00000480046 ENST00000419443

Gene Information

This gene is a member of a family of methyltransferases that share homology with but are distinct from the UbiE family of methyltransferases. Alternatively spliced variants which encode different protein isoforms have been described; however not all variants have been fully characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   METTL2B
Gene Name:   methyltransferase like 2B
Aliases:   FLJ11350, FLJ12760, METL, METTL2, METTL2A, PSENIP1
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000165055
Entrez:   55798
Chromosome Mapping:   7q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.590000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download