PrimePCR™ SYBR® Green Assay: KIAA1949, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040799

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KIAA1949, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KIAA1949, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038076
Assay Design:   Intron-spanning
Chromosome Location:   6:30647119-30652282question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000561623 ENST00000356183 ENST00000399199 ENST00000274853 ENST00000443517 ENST00000449705 ENST00000438815 ENST00000444206 ENST00000437121 ENST00000451544 ENST00000455935 ENST00000438945 ENST00000424970 ENST00000394833 ENST00000356736 ENST00000412189 ENST00000435929 ENST00000344721 ENST00000358102 ENST00000512865 ENST00000342437 ENST00000511528 ENST00000355292 ENST00000400554 ENST00000383573

Gene Information

Description Not Available

Gene Symbol:   KIAA1949
Gene Name:   KIAA1949
Aliases:   HKMT1098
RefSeq:   NT_167249.1 NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000146112
Entrez:   170954
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.010000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download