PrimePCR™ SYBR® Green Assay: CD99L2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040684

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CD99L2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CD99L2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037961
Assay Design:   Intron-spanning
Chromosome Location:   X:149937393-149938824question
Amplicon Length:   200
Splice Variants Targeted:   ENST00000336798 ENST00000563459 ENST00000564501 ENST00000563232 ENST00000449606 ENST00000309263 ENST00000450649 ENST00000520467 ENST00000457346 ENST00000333677 ENST00000300482 ENST00000397928 ENST00000300481 ENST00000370377 ENST00000466436 ENST00000355149 ENST00000437787 ENST00000361170 ENST00000597984 ENST00000601765 ENST00000600347 ENST00000596721

Gene Information

This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants. [provided by RefSeq May 2010]

Gene Symbol:   CD99L2
Gene Name:   CD99 molecule-like 2
Aliases:   CD99B, DKFZp761H2024, MIC2L1
RefSeq:   NC_000023.10 NG_012551.1 NT_167198.1 NG_021320.1
Ensembl:   ENSG00000102181
Entrez:   83692
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.560000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download