PrimePCR™ SYBR® Green Assay: PKM2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040657

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PKM2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PKM2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037934
Assay Design:   Intron-spanning
Chromosome Location:   15:72509750-72511317question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000565184 ENST00000335181 ENST00000565154 ENST00000568459 ENST00000561609 ENST00000564178 ENST00000562997 ENST00000567087 ENST00000566809 ENST00000319622 ENST00000389093 ENST00000243077 ENST00000533050 ENST00000360194 ENST00000369144 ENST00000541549 ENST00000205636

Gene Information

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq May 2011]

Gene Symbol:   PKM2
Gene Name:   pyruvate kinase, muscle
Aliases:   CTHBP, MGC3932, OIP3, PK3, PKM, TCB, THBP1
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000067225
Entrez:   5315
Chromosome Mapping:   15q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.670000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download