PrimePCR™ SYBR® Green Assay: NFYC, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040655

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NFYC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NFYC, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037932
Assay Design:   Intron-spanning
Chromosome Location:   1:41204567-41213250question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000296870 ENST00000286827 ENST00000541036 ENST00000344731 ENST00000269489 ENST00000373091 ENST00000250489 ENST00000398020 ENST00000368485 ENST00000344086 ENST00000427410 ENST00000447388 ENST00000425457 ENST00000453631 ENST00000456393 ENST00000372654 ENST00000534399 ENST00000372653 ENST00000372669 ENST00000372652 ENST00000372651 ENST00000531464 ENST00000440226 ENST00000525290 ENST00000416859 ENST00000308733

Gene Information

This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein subunit C forms a tight dimer with the B subunit a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Dec 2008]

Gene Symbol:   NFYC
Gene Name:   nuclear transcription factor Y, gamma
Aliases:   CBF-C, CBFC, DKFZp667G242, FLJ45775, H1TF2A, HAP5, HSM, NF-YC
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000066136
Entrez:   4802
Chromosome Mapping:   1p32,1p32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.740000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download