PrimePCR™ SYBR® Green Assay: PPP6R3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040622

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PPP6R3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PPP6R3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037899
Assay Design:   Intron-spanning
Chromosome Location:   11:68312341-68315611question
Amplicon Length:   199
Splice Variants Targeted:   ENST00000393800 ENST00000529907 ENST00000534534 ENST00000524845 ENST00000265637 ENST00000524904 ENST00000393801 ENST00000265636 ENST00000529710 ENST00000527403 ENST00000531244 ENST00000393799

Gene Information

Protein phosphatase regulatory subunits such as SAPS3 modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity recruiting substrates and determining the intracellular localization of the holoenzyme. SAPS3 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C; MIM 612725) (Stefansson and Brautigan 2006 [PubMed 16769727]).[supplied by OMIM Nov 2010]

Gene Symbol:   PPP6R3
Gene Name:   protein phosphatase 6, regulatory subunit 3
Aliases:   C11orf23, DKFZp781E17107, DKFZp781E2374, DKFZp781O2362, FLJ11058, FLJ43065, KIAA1558, MGC125711, MGC125712, PP6R3, SAP190, SAPL, SAPLa, SAPS3
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000110075
Entrez:   55291
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.580000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download