PrimePCR™ SYBR® Green Assay: ZNF727, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040274

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF727, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF727, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037551
Assay Design:   Intron-spanning
Chromosome Location:   7:63506000-63529894question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000234142 ENST00000432985 ENST00000381486 ENST00000509152 ENST00000526473 ENST00000261875 ENST00000458626 ENST00000525694 ENST00000550760 ENST00000423484 ENST00000355095 ENST00000587461 ENST00000590766 ENST00000540806 ENST00000450683 ENST00000587452 ENST00000545006 ENST00000443905 ENST00000591366 ENST00000592160 ENST00000418063 ENST00000596053 ENST00000597086 ENST00000596143 ENST00000596367 ENST00000601416 ENST00000291770 ENST00000600692 ENST00000599296 ENST00000311048 ENST00000598331 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF727
Gene Name:   zinc finger protein 727
Aliases:   Not Available
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000257482
Entrez:   442319
Chromosome Mapping:   7q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.470000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download