PrimePCR™ SYBR® Green Assay: GAGE12I, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040218

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GAGE12I, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GAGE12I, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037495
Assay Design:   Intron-spanning
Chromosome Location:   X:49221267-49223893question
Amplicon Length:   157
Splice Variants Targeted:   ENST00000407599 ENST00000442437 ENST00000381751 ENST00000402590 ENST00000404720 ENST00000415752 ENST00000381708 ENST00000381725 ENST00000420398 ENST00000405679 ENST00000381698 ENST00000440137 ENST00000445148 ENST00000381722 ENST00000362097 ENST00000381709 ENST00000376150 ENST00000244513 ENST00000305273 ENST00000594366 ENST00000597432 ENST00000593333 ENST00000600217 ENST00000600913 ENST00000593608 ENST00000601535 ENST00000601798 ENST00000596395 ENST00000593328 ENST00000595947 ENST00000601742

Gene Information

This gene belongs to a family of genes that are expressed in a variety of tumors but not in normal tissues except for the testis. The sequences of the family members are highly related but differ by scattered nucleotide substitutions. The antigenic peptide YYWPRPRRY which is also encoded by several other family members is recognized by autologous cytolytic T lymphocytes. [provided by RefSeq Jul 2008]

Gene Symbol:   GAGE12I
Gene Name:   G antigen 12I
Aliases:   AL4, CT4.7, GAGE12F, GAGE12G, GAGE7, GAGE7B
RefSeq:   NC_000023.10 NT_079573.4
Ensembl:   ENSG00000241465
Entrez:   26748
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 36.690000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download