PrimePCR™ SYBR® Green Assay: LSM2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040038

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LSM2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LSM2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037315
Assay Design:   Intron-spanning
Chromosome Location:   6:31766257-31773915question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000254508 ENST00000534751 ENST00000445041 ENST00000555262 ENST00000449574 ENST00000383391 ENST00000371623 ENST00000296474 ENST00000344206 ENST00000361216 ENST00000447527 ENST00000375661 ENST00000432122 ENST00000333461 ENST00000309664 ENST00000379709 ENST00000394323 ENST00000308580 ENST00000434125 ENST00000424975 ENST00000455705 ENST00000367603 ENST00000012134 ENST00000367604

Gene Information

This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 19. [provided by RefSeq Nov 2011]

Gene Symbol:   LSM2
Gene Name:   LSM2 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Aliases:   C6orf28, G7b, YBL026W, snRNP
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000204392
Entrez:   57819
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.060000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download