This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0039972
PrimePCR™ PreAmp for SYBR® Green Assay: GTF2IRD2, Human
PrimePCR™ Template for SYBR® Green Assay: GTF2IRD2, Human
This gene is unusual in that its coding sequence is mostly derived from Charlie8 repeat elements. However there is mRNA and EST evidence to suggest that this gene is transcribed and the encoded protein has a homolog in mouse with which it shares 78% sequence identity. The exact function of this gene product is not known. It is inferred to be a transcription factor based on the presence of GTF2I-like repeats (containing helix-loop-helix motifs) also found in other proteins such as GTF2IRD1 and GTF2I. These three genes are clustered together on chr 7q11.23. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.