PrimePCR™ SYBR® Green Assay: RGPD6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039907

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RGPD6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RGPD6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037184
Assay Design:   Intron-spanning
Chromosome Location:   2:111325541-111327278question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000304514 ENST00000409886 ENST00000354986 ENST00000408999 ENST00000283195 ENST00000393283 ENST00000430736 ENST00000016946 ENST00000432606 ENST00000272454 ENST00000329516 ENST00000330331 ENST00000446930 ENST00000455695 ENST00000302558 ENST00000409750 ENST00000330575 ENST00000559485 ENST00000409776 ENST00000398193 ENST00000327544 ENST00000420840 ENST00000398146

Gene Information

Description Not Available

Gene Symbol:   RGPD6
Gene Name:   RANBP2-like and GRIP domain containing 6
Aliases:   MGC190070, RGP6, RGPD5, RGPD7, RanBP2L1, RanBP2L2
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000183054
Entrez:   729540
Chromosome Mapping:   2q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.890000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download