PrimePCR™ SYBR® Green Assay: LGALS7B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039893

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: LGALS7B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LGALS7B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037170
Assay Design:   Intron-spanning
Chromosome Location:   19:39279855-39281450question
Amplicon Length:   199
Splice Variants Targeted:   ENST00000418477 ENST00000423043 ENST00000383214 ENST00000383213 ENST00000361360 ENST00000485511 ENST00000394110 ENST00000472118 ENST00000470467 ENST00000382363 ENST00000215798 ENST00000420843 ENST00000294618 ENST00000319867 ENST00000455915 ENST00000370069 ENST00000266068 ENST00000370077 ENST00000252137 ENST00000262879 ENST00000397042 ENST00000397040 ENST00000397038 ENST00000258796 ENST00000407643 ENST00000403167 ENST00000349466 ENST00000370186 ENST00000393842 ENST00000359149 ENST00000263519 ENST

Gene Information

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridization studies indicate that this lectin is specifically expressed in keratinocytes and found mainly in stratified squamous epithelium. A duplicate copy of this gene (GeneID:3963) is found adjacent to but on the opposite strand on chromosome 19. [provided by RefSeq Jul 2008]

Gene Symbol:   LGALS7B
Gene Name:   lectin, galactoside-binding, soluble, 7B
Aliases:   GAL7, Gal-7, HKL-14, LGALS7, MGC75149, MGC88745, PI7
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000178934
Entrez:   653499
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.710000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download