PrimePCR™ SYBR® Green Assay: LGALS9C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039870

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037147
Assay Design:   Intron-spanning
Chromosome Location:   17:18380155-18387251question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000395473 ENST00000302228 ENST00000313648 ENST00000577392 ENST00000310394 ENST00000413914 ENST00000319815 ENST00000522405 ENST00000521807 ENST00000309188 ENST00000439351 ENST00000581545 ENST00000582333 ENST00000328114 ENST00000583322 ENST00000584941 ENST00000301149 ENST00000423676 ENST00000324290

Gene Information

This gene was initially thought to represent a pseudogene of galectin 9; however this transcript has good exon-intron structure and encodes a predicted protein of the same size as and highly similar to galectin 9. This gene is one of two similar loci on chromosome 17p similar to galectin 9 and now thought to be protein-encoding. This gene is the more telomeric gene. [provided by RefSeq Jul 2008]

Gene Symbol:   LGALS9C
Gene Name:   lectin, galactoside-binding, soluble, 9C
Aliases:   MGC125972
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000171916
Entrez:   654346
UniGene:   Hs.462402
Chromosome Mapping:   17p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.100000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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