PrimePCR™ SYBR® Green Assay: DPCR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039852

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: DPCR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DPCR1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037129
Assay Design:   Intron-spanning
Chromosome Location:   6:30908767-30916355question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000251268 ENST00000412678 ENST00000274853 ENST00000409745 ENST00000330655 ENST00000443517 ENST00000296452 ENST00000394532 ENST00000354852 ENST00000457787 ENST00000438815 ENST00000514226 ENST00000429814 ENST00000437121 ENST00000455935 ENST00000412189 ENST00000433387 ENST00000266182 ENST00000440475 ENST00000281928 ENST00000549786 ENST00000311955 ENST00000398405 ENST00000433957 ENST00000291532 ENST00000380399 ENST00000548730 ENST00000372117 ENST00000440770 ENST00000400554 ENST00000547450 ENST

Gene Information

Description Not Available

Gene Symbol:   DPCR1
Gene Name:   diffuse panbronchiolitis critical region 1
Aliases:   MGC126710, MGC126712, PBLT
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000168631
Entrez:   135656
Chromosome Mapping:   6p21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.310000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download