PrimePCR™ SYBR® Green Assay: CASP5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039295

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0036571
Assay Design:   Intron-spanning
Chromosome Location:   11:104866508-104868173question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000260315 ENST00000444749 ENST00000526056 ENST00000531367 ENST00000393141 ENST00000418434 ENST00000393139

Gene Information

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits large and small that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max a central component of the Myc/Max/Mad transcription regulation network important for cell growth differentiation and apoptosis is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq Aug 2010]

Gene Symbol:   CASP5
Gene Name:   caspase 5, apoptosis-related cysteine peptidase
Aliases:   ICE(rel)III, ICEREL-III, ICH-3, MGC141966
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000137757
Entrez:   838
UniGene:   Hs.213327
Chromosome Mapping:   11q22.2-q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.880000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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