PrimePCR™ SYBR® Green Assay: MS4A4A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039137

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MS4A4A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MS4A4A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0036413
Assay Design:   Intron-spanning
Chromosome Location:   11:60064706-60068526question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000532114 ENST00000337908 ENST00000355131 ENST00000395016

Gene Information

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features similar intron/exon splice boundaries and display unique expression patterns in hematopoietic cells and nonlymphoid tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   MS4A4A
Gene Name:   membrane-spanning 4-domains, subfamily A, member 4
Aliases:   4SPAN1, CD20-L1, CD20L1, MGC22311, MS4A4, MS4A7
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000110079
Entrez:   51338
Chromosome Mapping:   11q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.680000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download