PrimePCR™ SYBR® Green Assay: CBLN4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039042

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0036318
Assay Design:   Intron-spanning
Chromosome Location:   20:54575844-54579014question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000064571

Gene Information

Cerebellin is a sixteen aa peptide found mainly in the adrenal medulla where it has been shown to have a neuromodulatory function. Cerebellin is derived from precerebellin a protein with sequence similarity to the noncollagen domain of complement component C1qB. The protein encoded by this gene is a glycoprotein which shares sequence similarity with precerebellin. [provided by RefSeq Jul 2008]

Gene Symbol:   CBLN4
Gene Name:   cerebellin 4 precursor
Aliases:   CBLNL1
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000054803
Entrez:   140689
UniGene:   Hs.126141
Chromosome Mapping:   20q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.020000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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