PrimePCR™ SYBR® Green Assay: SLC26A6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033842

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC26A6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC26A6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0023669
Assay Design:   Intron-spanning
Chromosome Location:   3:48665958-48667138question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000420764 ENST00000395550 ENST00000383733 ENST00000337000 ENST00000455886 ENST00000447978 ENST00000358747

Gene Information

This gene belongs to the solute carrier 26 family whose members encode anion transporter proteins. This particular family member encodes a protein involved in transporting chloride oxalate sulfate and bicarbonate. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been described but the full-length nature of some of these variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   SLC26A6
Gene Name:   solute carrier family 26, member 6
Aliases:   DKFZp586E1422
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000225697
Entrez:   65010
Chromosome Mapping:   3p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.920000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download