This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0033842
PrimePCR™ PreAmp for SYBR® Green Assay: SLC26A6, Human
PrimePCR™ Template for SYBR® Green Assay: SLC26A6, Human
This gene belongs to the solute carrier 26 family whose members encode anion transporter proteins. This particular family member encodes a protein involved in transporting chloride oxalate sulfate and bicarbonate. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been described but the full-length nature of some of these variants has not been determined. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.