PrimePCR™ SYBR® Green Assay: BCAT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033683

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BCAT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BCAT1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0023094
Assay Design:   Intron-spanning
Chromosome Location:   12:24970908-24982831question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000261192 ENST00000538118 ENST00000342945 ENST00000539282 ENST00000539780

Gene Information

This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq May 2010]

Gene Symbol:   BCAT1
Gene Name:   branched chain amino-acid transaminase 1, cytosolic
Aliases:   BCATC, BCT1, DKFZp686E12175, ECA39, MECA39, PNAS121, PP18
RefSeq:   NC_000012.11 NT_009714.17 NG_008170.1
Ensembl:   ENSG00000060982
Entrez:   586
Chromosome Mapping:   12p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 36.990000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download