PrimePCR™ SYBR® Green Assay: ZNF839, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0023077
Assay Design:   Intron-spanning
Chromosome Location:   14:102800944-102802165question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000442396 ENST00000262236 ENST00000398436

Gene Information

Description Not Available

Gene Symbol:   ZNF839
Gene Name:   zinc finger protein 839
Aliases:   C14orf131, FLJ11132
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000022976
Entrez:   55778
UniGene:   Hs.106005
Chromosome Mapping:   14q32.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.660000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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