This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0033608
PrimePCR™ PreAmp for SYBR® Green Assay: CSNK1E, Human
PrimePCR™ Template for SYBR® Green Assay: CSNK1E, Human
The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family whose members have been implicated in the control of cytoplasmic and nuclear processes including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins including itself. This protein has been shown to phosphorylate period a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.