PrimePCR™ SYBR® Green Assay: PFKFB3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033497

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0022601
Assay Design:   Intron-spanning
Chromosome Location:   10:6264904-6266163question
Amplicon Length:   129
Splice Variants Targeted:   ENST00000317350 ENST00000360521 ENST00000379775 ENST00000358499 ENST00000450232 ENST00000540253 ENST00000379782 ENST00000379784 ENST00000379781 ENST00000444592 ENST00000379789 ENST00000379785

Gene Information

Description Not Available

Gene Symbol:   PFKFB3
Gene Name:   6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Aliases:   FLJ37326, IPFK2, PFK2
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000170525
Entrez:   5209
UniGene:   Hs.195471
Chromosome Mapping:   10p15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.860000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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