PrimePCR™ SYBR® Green Assay: YY1AP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: YY1AP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: YY1AP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0022503
Assay Design:   Intron-spanning
Chromosome Location:   1:155644841-155646441question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000359205 ENST00000355499 ENST00000311573 ENST00000347088 ENST00000361831 ENST00000368340 ENST00000295566 ENST00000368330 ENST00000407221 ENST00000404643 ENST00000405763 ENST00000443231 ENST00000454523 ENST00000368339 ENST00000438245

Gene Information

The encoded gene product presumably interacts with YY1 protein; however its exact function is not known. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   YY1AP1
Gene Name:   YY1 associated protein 1
Aliases:   FLJ10875, FLJ13914, HCCA1, HCCA2, YAP, YY1AP
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000163374
Entrez:   55249
Chromosome Mapping:   1q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.770000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download