PrimePCR™ SYBR® Green Assay: PDIA6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033410

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PDIA6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PDIA6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0022393
Assay Design:   Intron-spanning
Chromosome Location:   2:10937842-10942759question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000272227 ENST00000404371 ENST00000404824 ENST00000381611 ENST00000540494

Gene Information

Protein disulfide isomerases (EC 5.3.4.1) such as PDIA6 are endoplasmic reticulum (ER) resident proteins that catalyze formation reduction and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins (Hayano and Kikuchi 1995 [PubMed 7590364]).[supplied by OMIM Mar 2008]

Gene Symbol:   PDIA6
Gene Name:   protein disulfide isomerase family A, member 6
Aliases:   ERP5, P5, TXNDC7
RefSeq:   NC_000002.11 NT_005334.16
Ensembl:   ENSG00000143870
Entrez:   10130
Chromosome Mapping:   2p25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.480000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download