PrimePCR™ SYBR® Green Assay: PRKD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033266

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PRKD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PRKD1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0022040
Assay Design:   Intron-spanning
Chromosome Location:   14:30135322-30194788question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000331968 ENST00000415220 ENST00000549503

Gene Information

PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions including membrane receptor signaling transport at the Golgi protection from oxidative stress at the mitochondria gene transcription and regulation of cell shape motility and adhesion (summary by Eiseler et al. 2009 [PubMed 19329994]).[supplied by OMIM Nov 2010]

Gene Symbol:   PRKD1
Gene Name:   protein kinase D1
Aliases:   PKC-MU, PKCM, PKD, PRKCM
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000184304
Entrez:   5587
Chromosome Mapping:   14q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.190000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download