PrimePCR™ SYBR® Green Assay: ICA1L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ICA1L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ICA1L, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021517
Assay Design:   Intron-spanning
Chromosome Location:   2:203690438-203693617question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000392237 ENST00000358299 ENST00000420558 ENST00000425178 ENST00000450143 ENST00000435143 ENST00000419460 ENST00000441547 ENST00000412210 ENST00000457524 ENST00000432273 ENST00000416760 ENST00000454326 ENST00000411681

Gene Information

Description Not Available

Gene Symbol:   ICA1L
Gene Name:   islet cell autoantigen 1,69kDa-like
Aliases:   ALS2CR14, ALS2CR15, DKFZp434E1919, MGC138440
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000163596
Entrez:   130026
Chromosome Mapping:   2q33.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.700000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download