PrimePCR™ SYBR® Green Assay: MEGF10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033032

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MEGF10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MEGF10, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021398
Assay Design:   Intron-spanning
Chromosome Location:   5:126738320-126746131question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000503335 ENST00000508365 ENST00000418761 ENST00000274473

Gene Information

This gene encodes a member of the multiple epidermal growth factor-like domains protein family. The encoded protein plays a role in cell adhesion motility and proliferation and is a critical mediator of apoptotic cell phagocytosis as well as amyloid-beta peptide uptake in the brain. Expression of this gene may be associated with schizophrenia and mutations in this gene are a cause of early-onset myopathy areflexia respiratory distress and dysphagia (EMARDD). Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq Feb 2012]

Gene Symbol:   MEGF10
Gene Name:   multiple EGF-like-domains 10
Aliases:   DKFZp781K1852, FLJ41574, KIAA1780
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000145794
Entrez:   84466
Chromosome Mapping:   5q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.090000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download