PrimePCR™ SYBR® Green Assay: IQGAP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033015

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: IQGAP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IQGAP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021346
Assay Design:   Intron-spanning
Chromosome Location:   15:91030293-91034615question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000268182

Gene Information

This gene encodes a member of the IQGAP family. The protein contains four IQ domains one calponin homology domain one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton with cell adhesion molecules and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq Jul 2008]

Gene Symbol:   IQGAP1
Gene Name:   IQ motif containing GTPase activating protein 1
Aliases:   HUMORFA01, KIAA0051, SAR1, p195
RefSeq:   NC_000015.9 NT_010274.17
Ensembl:   ENSG00000140575
Entrez:   8826
Chromosome Mapping:   15q26.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.070000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download