PrimePCR™ SYBR® Green Assay: SRSF11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0032929

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SRSF11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SRSF11, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021141
Assay Design:   Intron-spanning
Chromosome Location:   1:70687492-70694197question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000370951 ENST00000370950 ENST00000395136 ENST00000405432 ENST00000436161 ENST00000454435

Gene Information

This gene encodes 54-kD nuclear protein that contains an arginine/serine-rich region similar to segments found in pre-mRNA splicing factors. Although the function of this protein is not yet known structure and immunolocalization data suggest that it may play a role in pre-mRNA processing. Alternative splicing results in multiple transcript variants encoding different proteins. In addition a pseudogene of this gene has been found on chromosome 12.[provided by RefSeq Sep 2010]

Gene Symbol:   SRSF11
Gene Name:   serine/arginine-rich splicing factor 11
Aliases:   DKFZp686M13204, FLJ18226, NET2, SFRS11, dJ677H15.2, p54
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000116754
Entrez:   9295
Chromosome Mapping:   1p31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.860000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download