PrimePCR™ SYBR® Green Assay: AHSA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021003
Assay Design:   Intron-spanning
Chromosome Location:   14:77929024-77931005question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000555133 ENST00000216479 ENST00000535854 ENST00000553374

Gene Information

Description Not Available

Gene Symbol:   AHSA1
Gene Name:   AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
Aliases:   AHA1, C14orf3, p38
RefSeq:   NC_000014.8 NG_023421.1 NT_026437.12
Ensembl:   ENSG00000100591
Entrez:   10598
Chromosome Mapping:   14q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.460000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download