PrimePCR™ SYBR® Green Assay: MRE11A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0020877
Assay Design:   Intron-spanning
Chromosome Location:   11:94212867-94219132question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000323929 ENST00000407439 ENST00000323977 ENST00000393241 ENST00000540013 ENST00000536754

Gene Information

This gene encodes a nuclear protein involved in homologous recombination telomere length maintenance and DNA double-strand break repair. By itself the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   MRE11A
Gene Name:   MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
Aliases:   ATLD, HNGS1, MRE11, MRE11B
RefSeq:   NC_000011.9 NT_167190.1 NG_007261.1
Ensembl:   ENSG00000020922
Entrez:   4361
UniGene:   Hs.192649
Chromosome Mapping:   11q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.920000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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