PrimePCR™ SYBR® Green Assay: MUC6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MUC6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MUC6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0020103
Assay Design:   Intron-spanning
Chromosome Location:   11:1031613-1031859question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000421673 ENST00000525923

Gene Information

The MUC6 gene encodes gastric mucin a secreted glycoprotein that plays an essential role in epithelial cytoprotection from acid proteases pathogenic microorganisms and mechanical trauma in the gastrointestinal tract (summary by Toribara et al. 1993 [PubMed 7680650]).[supplied by OMIM Dec 2010]

Gene Symbol:   MUC6
Gene Name:   mucin 6, oligomeric mucus/gel-forming
Aliases:   MUC-6
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000184956
Entrez:   4588
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.330000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download