PrimePCR™ SYBR® Green Assay: DDX27, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031963

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DDX27, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DDX27, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018618
Assay Design:   Intron-spanning
Chromosome Location:   20:47838017-47839546question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000371764 ENST00000535160

Gene Information

DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation nuclear and mitochondrial splicing and ribosome and spliceosome assembly. Based on their distribution patterns some members of this family are believed to be involved in embryogenesis spermatogenesis and cellular growth and division. This gene encodes a DEAD box protein the function of which has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   DDX27
Gene Name:   DEAD (Asp-Glu-Ala-Asp) box polypeptide 27
Aliases:   DKFZp667N057, DRS1, FLJ12917, FLJ20596, FLJ22238, MGC1018, MGC163147, PP3241, RHLP, Rrp3p, dJ686N3.1
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000124228
Entrez:   55661
Chromosome Mapping:   20q13.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.060000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download